Liberal frontbencher Andrew Hastie has lashed out at “lying cowards” Pauline Hanson and Barnaby Joyce over a One Nation cartoon labelling him a “traitor”.

One Nation’s leader has refused to take down the nine-minute animated video that she has pinned to the top of her X account.

The Don’t Be Hastie episode features Hastie complaining to Prime Minister Anthony Albanese that people are calling him a traitor online.

“All I did was testify against Ben Roberts-Smith and support your evil hate speech legislation,” the cartoon Hastie tells the PM.

The former SAS soldier, who was deployed twice to Afghanistan, called on One Nation to delete the cartoon or own up to it in parliament.

The Western Australian MP accused One Nation of politicising the court proceedings of accused war criminal Roberts-Smith and “trashing” his own service record.

“I would say that Pauline Hanson and Barnaby Joyce are lying cowards, and I’ll say it again so there’s no ambiguity,” he told News24.

“They are lying cowards.”

Hastie said the cartoon called him a traitor eight times.

“When you start calling me a traitor to my own country, then we have a big, big problem,” he said.

Hanson rejected the claims, saying she was gobsmacked.

“I’m not calling him a traitor to his country,” she told News24 on Wednesday.

“I have not said that. It is a satirical cartoon, for Christ’s sake.

“Barnaby Joyce was classified as a beetroot. He’s now part of the party. Did he jump up and down about it? So stop making a mountain out of molehill.”

There has been tension between Hastie and Hanson since he spoke out against Roberts-Smith. Hastie also gave evidence in court against Roberts-Smith.

Hanson’s closest supporter, billionaire Gina Rinehart, has backed Roberts-Smith, and has named a planned housing complex for veterans in his honour.

Earlier, Hanson told The Nightly newspaper she would define Hastie as “a wanker”.

Joyce on Wednesday denied knowledge of the cartoon.

“I dearly hope that nobody is threatening Andrew or his family, and certainly not premising [threats] on anything to do with One Nation,” he said.

“It’s got nothing to do with One Nation.”

Hastie emailed his supporters earlier this week declaring that he would “not take any more slander from One Nation”.

“I believe in the rule of law, so I complied with the subpoena rather than be found in contempt. I gave evidence under oath, before God,” he said.

“For that, I’ve been called a rat, a traitor, a dog c—, and many other things. People I trusted have walked away from me or failed to defend me.”

Hastie said that “cowards who have never served a day in the Australian Defence Force” were attacking his service record, “my integrity, and my loyalty to Australia”.

-with AAP